BD HorizonTM BUV661 Rat Anti-Mouse CD4

Description

Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BUV661 Rat IgG2b, kappa Isotype Control Cat. No. 612978; Left Plot or BD Horizon BUV661 Rat Anti-Mouse CD4 antibody Cat. No. 612974; Right Plot at 0.5 mug/test. The two-color pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BUV661 Rat IgG2b, kappa Isotype Control Cat. No. 612978; Left Plot or BD Horizon BUV661 Rat Anti-Mouse CD4 antibody Cat. No. 612974; Right Plot at 0.5 mug/test. The two-color pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BUV661 Rat IgG2b, kappa Isotype Control Cat. No. 612978; Left Plot or BD Horizon BUV661 Rat Anti-Mouse CD4 antibody Cat. No. 612974; Right Plot at 0.5 mug/test. The two-color pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BUV661 Rat IgG2b, kappa Isotype Control Cat. No. 612978; Left Plot or BD Horizon BUV661 Rat Anti-Mouse CD4 antibody Cat. No. 612974; Right Plot at 0.5 mug/test. The two-color pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BUV661 Rat IgG2b, kappa Isotype Control Cat. No. 612978; Left Plot or BD Horizon BUV661 Rat Anti-Mouse CD4 antibody Cat. No. 612974; Right Plot at 0.5 mug/test. The two-color pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BUV661 Rat IgG2b, kappa Isotype Control Cat. No. 612978; Left Plot or BD Horizon BUV661 Rat Anti-Mouse CD4 antibody Cat. No. 612974; Right Plot at 0.5 mug/test. The two-color pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.