BD HorizonTM BUV661 Streptavidin

Description

Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV661 Streptavidin Cat. No. 612979 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV661 Streptavidin Cat. No. 612979 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV661 Streptavidin Cat. No. 612979 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV661 Streptavidin Cat. No. 612979 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV661 Streptavidin Cat. No. 612979 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . The cells were then washed and secondarily stained with BD Horizon BUV661 Streptavidin Cat. No. 612979 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.