BD HorizonTM BUV737 Hamster Anti-Mouse CD11c

Description

Multicolor flow cytometric analysis of CD11c expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon BUV737 Hamster IgG2, lambda1 Isotype Control Cat. No. 612822; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD11c antibody Cat. No. 569236; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon BUV737 Hamster IgG2, lambda1 Isotype Control Cat. No. 612822; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD11c antibody Cat. No. 569236; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon BUV737 Hamster IgG2, lambda1 Isotype Control Cat. No. 612822; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD11c antibody Cat. No. 569236; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon BUV737 Hamster IgG2, lambda1 Isotype Control Cat. No. 612822; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD11c antibody Cat. No. 569236; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon BUV737 Hamster IgG2, lambda1 Isotype Control Cat. No. 612822; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD11c antibody Cat. No. 569236; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E antibody Cat. No. 562367 and with either BD Horizon BUV737 Hamster IgG2, lambda1 Isotype Control Cat. No. 612822; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD11c antibody Cat. No. 569236; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.