BD HorizonTM BUV737 Mouse Anti-EOMES

Description

Multicolor flow cytometric analysis of EOMES expression in human peripheral blood lymphocytes. Peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Cells were stained with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428/ 562429 and either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-EOMES antibody Cat. No. 567170; Right Plot at 1 mug/test. The bivariate pseudocolor density plots showing EOMES expression or Ig isotype control staining versus CD8 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of EOMES expression in mouse splenic leucocyte populations. Spleen cells from C57BL/6 mice were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set. Cells were stained intracellularly with BD Horizon BV421 Rat anti-mouse CD335 NKp46 antibody Cat.No. 564065 and either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BUV737 Mouse Anti-EOMES antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plots showing EOMES expression or Ig isotype control staining versus CD355 NKp46 were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of EOMES expression in human peripheral blood lymphocytes. Peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Cells were stained with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428/ 562429 and either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-EOMES antibody Cat. No. 567170; Right Plot at 1 mug/test. The bivariate pseudocolor density plots showing EOMES expression or Ig isotype control staining versus CD8 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of EOMES expression in mouse splenic leucocyte populations. Spleen cells from C57BL/6 mice were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set. Cells were stained intracellularly with BD Horizon BV421 Rat anti-mouse CD335 NKp46 antibody Cat.No. 564065 and either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BUV737 Mouse Anti-EOMES antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plots showing EOMES expression or Ig isotype control staining versus CD355 NKp46 were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of EOMES expression in human peripheral blood lymphocytes. Peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Cells were stained with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428/ 562429 and either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-EOMES antibody Cat. No. 567170; Right Plot at 1 mug/test. The bivariate pseudocolor density plots showing EOMES expression or Ig isotype control staining versus CD8 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of EOMES expression in mouse splenic leucocyte populations. Spleen cells from C57BL/6 mice were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set. Cells were stained intracellularly with BD Horizon BV421 Rat anti-mouse CD335 NKp46 antibody Cat.No. 564065 and either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BUV737 Mouse Anti-EOMES antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plots showing EOMES expression or Ig isotype control staining versus CD355 NKp46 were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.