BD HorizonTM BUV737 Mouse Anti-Human IL-17A

Description

Two-color flow cytometric analysis of IL-17A expression in stimulated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-Human IL-17A antibody Cat. No. 570068/570149; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of IL-17A expression in stimulated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-Human IL-17A antibody Cat. No. 570068/570149; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of IL-17A expression in stimulated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-Human IL-17A antibody Cat. No. 570068/570149; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of IL-17A expression in stimulated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-Human IL-17A antibody Cat. No. 570068/570149; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of IL-17A expression in stimulated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-Human IL-17A antibody Cat. No. 570068/570149; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of IL-17A expression in stimulated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with APC Mouse Anti-Human CD4 antibody Cat. No. 561841 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plot or BD Horizon BUV737 Mouse Anti-Human IL-17A antibody Cat. No. 570068/570149; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.