BD HorizonTM BUV737 Mouse Anti-Human KLRG1

Description

Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 564963; Top Plots and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plots or BD Horizon BUV737 Mouse Anti-Human KLRG1 antibody Cat. No. 571548/571590; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 564963; Top Plots and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plots or BD Horizon BUV737 Mouse Anti-Human KLRG1 antibody Cat. No. 571548/571590; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon PE-CF594 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 564963; Top Plots and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plots or BD Horizon BUV737 Mouse Anti-Human KLRG1 antibody Cat. No. 571548/571590; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.