BD HorizonTM BUV737 Mouse Anti-Human TIM-3 (CD366)

Description

Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No.555516/561903 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plots or BD Horizon BUV737 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 568680; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of either TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leucocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No.555516/561903 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plots or BD Horizon BUV737 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 568680; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of either TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leucocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No.555516/561903 and with either BD Horizon BUV737 Mouse IgG1, kappa Isotype Control Cat. No. 612758; Left Plots or BD Horizon BUV737 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 568680; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of either TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leucocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.