BD HorizonTM BUV737 Rat Anti-Mouse Blimp-1

Description

Two-color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; Left Plot or BD Horizon BUV737 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567834; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype-stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; Left Plot or BD Horizon BUV737 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567834; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype-stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; Left Plot or BD Horizon BUV737 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567834; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype-stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; Left Plot or BD Horizon BUV737 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567834; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype-stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; Left Plot or BD Horizon BUV737 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567834; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype-stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; Left Plot or BD Horizon BUV737 Rat Anti-Mouse Blimp-1 antibody Cat. No. 567834; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype-stained cells data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.