BD HorizonTM BUV737 Rat Anti-Mouse Ly-6G

Description

Flow cytometric analysis of Ly-6G expression on Mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; dashed line histograms or BD Horizon BUV737 Rat Anti-Mouse Ly-6G antibody Cat. No. 568346; solid line histograms at 1.0 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expressed levels of Ly-6G or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoid Left Plot or myeloid Right Plot cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on Mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; dashed line histograms or BD Horizon BUV737 Rat Anti-Mouse Ly-6G antibody Cat. No. 568346; solid line histograms at 1.0 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expressed levels of Ly-6G or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoid Left Plot or myeloid Right Plot cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on Mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; dashed line histograms or BD Horizon BUV737 Rat Anti-Mouse Ly-6G antibody Cat. No. 568346; solid line histograms at 1.0 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expressed levels of Ly-6G or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoid Left Plot or myeloid Right Plot cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-6G expression on Mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; dashed line histograms or BD Horizon BUV737 Rat Anti-Mouse Ly-6G antibody Cat. No. 568346; solid line histograms at 1.0 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expressed levels of Ly-6G or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoid Left Plot or myeloid Right Plot cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-6G expression on Mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; dashed line histograms or BD Horizon BUV737 Rat Anti-Mouse Ly-6G antibody Cat. No. 568346; solid line histograms at 1.0 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expressed levels of Ly-6G or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoid Left Plot or myeloid Right Plot cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on Mouse bone-marrow leucocytes. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon BUV737 Rat IgG2a, kappa Isotype Control Cat. No. 612760; dashed line histograms or BD Horizon BUV737 Rat Anti-Mouse Ly-6G antibody Cat. No. 568346; solid line histograms at 1.0 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing the expressed levels of Ly-6G or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoid Left Plot or myeloid Right Plot cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.