BD HorizonTM BUV805 Hamster Anti-Mouse CD11c (Integrin alphaX)

Description

Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse I-A/I-E antibody Cat. No. 557000 and with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; Left Plot or BD Horizon BUV805 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569713; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse I-A/I-E antibody Cat. No. 557000 and with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; Left Plot or BD Horizon BUV805 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569713; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse I-A/I-E antibody Cat. No. 557000 and with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; Left Plot or BD Horizon BUV805 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569713; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse I-A/I-E antibody Cat. No. 557000 and with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; Left Plot or BD Horizon BUV805 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569713; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse I-A/I-E antibody Cat. No. 557000 and with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; Left Plot or BD Horizon BUV805 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569713; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse I-A/I-E antibody Cat. No. 557000 and with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; Left Plot or BD Horizon BUV805 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 569713; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.