BD HorizonTM BUV805 Hamster Anti-Mouse CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 expression on resting and activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Panel or activated Right Panel in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; dashed line histograms or BD Horizon BUV805 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 569610/569611; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Panel or activated Right Panel in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; dashed line histograms or BD Horizon BUV805 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 569610/569611; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Panel or activated Right Panel in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; dashed line histograms or BD Horizon BUV805 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 569610/569611; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Panel or activated Right Panel in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; dashed line histograms or BD Horizon BUV805 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 569610/569611; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Panel or activated Right Panel in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; dashed line histograms or BD Horizon BUV805 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 569610/569611; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Panel or activated Right Panel in culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Hamster IgG2, kappa Isotype Control Cat. No. 612910; dashed line histograms or BD Horizon BUV805 Hamster Anti-Mouse CD279 PD-1 antibody Cat. No. 569610/569611; solid line histograms at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD279 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.