BD HorizonTM BUV805 Mouse Anti-Bcl-6

Description

Multicolor flow cytometric analysis of Bcl-6 expression in mouse B lymphocytes. BALB/c mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , FITC Rat Anti-Mouse CD4 Cat. No. 553047 , Alexa Fluor 647 Hamster Anti-Mouse Fas/CD95 Cat. No. 563647 , and BD Horizon BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV805 Mouse Anti-Bcl-6 antibody. The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of Bcl-6 expression in mouse B lymphocytes. BALB/c mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , FITC Rat Anti-Mouse CD4 Cat. No. 553047 , Alexa Fluor 647 Hamster Anti-Mouse Fas/CD95 Cat. No. 563647 , and BD Horizon BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV805 Mouse Anti-Bcl-6 antibody. The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of Bcl-6 expression in mouse B lymphocytes. BALB/c mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , FITC Rat Anti-Mouse CD4 Cat. No. 553047 , Alexa Fluor 647 Hamster Anti-Mouse Fas/CD95 Cat. No. 563647 , and BD Horizon BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV805 Mouse Anti-Bcl-6 antibody. The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.