BD HorizonTM BUV805 Mouse Anti-Human CD152 (CTLA-4)

Description

Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335 and with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 569654/569655; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335 and with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 569654/569655; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335 and with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 569654/569655; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335 and with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 569654/569655; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335 and with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 569654/569655; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335 and with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 569654/569655; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software.