BD HorizonTM BUV805 Mouse Anti-Human CD223 (LAG-3)

Description

Multicolor flow cytometric analysis of CD223 LAG-3 expression on unstimulated Top Plots and stimulated Bottom Plots Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 567109/567108; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Recombinant Human IL-2 Protein Cat. No. 554603; 10 ng/mL . Unstimulated PBMC from the same donor and stimulated PBMC were stained with BD Horizon BB515 Mouse Anti-Human CD8 Cat. No. 564526; Left Plots , BD Horizon BV480 Mouse Anti-Human CD279 PD-1 Cat. No. 566112; Right Plots , and BD Horizon BUV805 Mouse Anti-Human CD223 LAG-3 Cat. No. 569664/569665 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD223 LAG-3 versus CD8 Left Plots , or CD223 LAG-3 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD223 LAG-3 expression on unstimulated Top Plots and stimulated Bottom Plots Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 567109/567108; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Recombinant Human IL-2 Protein Cat. No. 554603; 10 ng/mL . Unstimulated PBMC from the same donor and stimulated PBMC were stained with BD Horizon BB515 Mouse Anti-Human CD8 Cat. No. 564526; Left Plots , BD Horizon BV480 Mouse Anti-Human CD279 PD-1 Cat. No. 566112; Right Plots , and BD Horizon BUV805 Mouse Anti-Human CD223 LAG-3 Cat. No. 569664/569665 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD223 LAG-3 versus CD8 Left Plots , or CD223 LAG-3 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD223 LAG-3 expression on unstimulated Top Plots and stimulated Bottom Plots Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 567109/567108; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Recombinant Human IL-2 Protein Cat. No. 554603; 10 ng/mL . Unstimulated PBMC from the same donor and stimulated PBMC were stained with BD Horizon BB515 Mouse Anti-Human CD8 Cat. No. 564526; Left Plots , BD Horizon BV480 Mouse Anti-Human CD279 PD-1 Cat. No. 566112; Right Plots , and BD Horizon BUV805 Mouse Anti-Human CD223 LAG-3 Cat. No. 569664/569665 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD223 LAG-3 versus CD8 Left Plots , or CD223 LAG-3 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.