BD HorizonTM BUV805 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BUV805 Mouse IgG1, kappa Isotype Control Cat. No. 612897; Left Plots or BD Horizon BUV805 Mouse Anti-Ki-67 antibody Cat. No. 569635/569636; Right Plots at 1 mug/test and counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of 7-AAD staining versus Ki-67 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or MOLT-4 cells Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BUV805 Mouse IgG1, kappa Isotype Control Cat. No. 612897; Left Plots or BD Horizon BUV805 Mouse Anti-Ki-67 antibody Cat. No. 569635/569636; Right Plots at 1 mug/test and counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of 7-AAD staining versus Ki-67 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or MOLT-4 cells Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BUV805 Mouse IgG1, kappa Isotype Control Cat. No. 612897; Left Plots or BD Horizon BUV805 Mouse Anti-Ki-67 antibody Cat. No. 569635/569636; Right Plots at 1 mug/test and counterstained with BD Via-Probe Cat. No. 555815; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of 7-AAD staining versus Ki-67 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or MOLT-4 cells Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.