BD HorizonTM BUV805 Mouse Anti-Mouse CD45.2

Description

Flow cytometric analysis of CD45.2 expression on viable Mouse splenic leucocytes. Mouse splenic leucocytes from an SJL Mouse Left Panel or a C57BL/6 Mouse Right Panel were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; dashed line histograms or BD Horizon BUV805 Mouse Anti-Mouse CD45.2 antibody Cat. No 569200; solid line histograms at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD45.2 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.2 expression on viable Mouse splenic leucocytes. Mouse splenic leucocytes from an SJL Mouse Left Panel or a C57BL/6 Mouse Right Panel were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; dashed line histograms or BD Horizon BUV805 Mouse Anti-Mouse CD45.2 antibody Cat. No 569200; solid line histograms at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD45.2 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.2 expression on viable Mouse splenic leucocytes. Mouse splenic leucocytes from an SJL Mouse Left Panel or a C57BL/6 Mouse Right Panel were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; dashed line histograms or BD Horizon BUV805 Mouse Anti-Mouse CD45.2 antibody Cat. No 569200; solid line histograms at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD45.2 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.2 expression on viable Mouse splenic leucocytes. Mouse splenic leucocytes from an SJL Mouse Left Panel or a C57BL/6 Mouse Right Panel were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; dashed line histograms or BD Horizon BUV805 Mouse Anti-Mouse CD45.2 antibody Cat. No 569200; solid line histograms at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD45.2 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.2 expression on viable Mouse splenic leucocytes. Mouse splenic leucocytes from an SJL Mouse Left Panel or a C57BL/6 Mouse Right Panel were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; dashed line histograms or BD Horizon BUV805 Mouse Anti-Mouse CD45.2 antibody Cat. No 569200; solid line histograms at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD45.2 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.2 expression on viable Mouse splenic leucocytes. Mouse splenic leucocytes from an SJL Mouse Left Panel or a C57BL/6 Mouse Right Panel were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Mouse IgG2a, kappa Isotype Control Cat. No. 612904; dashed line histograms or BD Horizon BUV805 Mouse Anti-Mouse CD45.2 antibody Cat. No 569200; solid line histograms at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD45.2 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.