BD HorizonTM BUV805 Rat Anti-CD11b

Description

Multiparameter flow cytometric analysis of CD11b expression on mouse bone-marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD11b antibody Cat. No. 568345; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative bone marrow cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b expression on human peripheral blood leucocytes. Whole blood was stained with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD11b antibody Cat. No. 568345; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b expression on mouse bone-marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD11b antibody Cat. No. 568345; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative bone marrow cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD11b expression on human peripheral blood leucocytes. Whole blood was stained with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD11b antibody Cat. No. 568345; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD11b expression on mouse bone-marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD11b antibody Cat. No. 568345; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative bone marrow cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b expression on human peripheral blood leucocytes. Whole blood was stained with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Mouse Anti-Human CD11b antibody Cat. No. 568345; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.