BD HorizonTM BUV805 Rat Anti-Mouse CD3

Description

Two-color flow cytometric analysis of CD3 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD3 antibody Cat. No. 569192; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD3 antibody Cat. No. 569192; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD3 antibody Cat. No. 569192; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD3 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD3 antibody Cat. No. 569192; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD3 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD3 antibody Cat. No. 569192; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3 expression on Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon BUV805 Rat IgG2b, kappa Isotype Control Cat. No. 612901; Left Plot or BD Horizon BUV805 Rat Anti-Mouse CD3 antibody Cat. No. 569192; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.