BD HorizonTM BUV805 Rat Anti-Mouse IL-17A

Description

Multiparameter flow cytometric analysis of IL-17A expression in resting and stimulated Mouse EL-4 cells. Cells from the Mouse EL4 T lymphoma, ATCC TIB-39 cell line were either not stimulated Left Plot or stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724; Right Plot . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV805 Rat Anti-Mouse IL-17A antibody Cat. No. 570075/570156 at 0.5 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plots showing the correlated expression of IL-17A in resting Left Plot or stimulated EL-4 cells Right Plot versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact EL-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of IL-17A expression in resting and stimulated Mouse EL-4 cells. Cells from the Mouse EL4 T lymphoma, ATCC TIB-39 cell line were either not stimulated Left Plot or stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724; Right Plot . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV805 Rat Anti-Mouse IL-17A antibody Cat. No. 570075/570156 at 0.5 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plots showing the correlated expression of IL-17A in resting Left Plot or stimulated EL-4 cells Right Plot versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact EL-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of IL-17A expression in resting and stimulated Mouse EL-4 cells. Cells from the Mouse EL4 T lymphoma, ATCC TIB-39 cell line were either not stimulated Left Plot or stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724; Right Plot . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV805 Rat Anti-Mouse IL-17A antibody Cat. No. 570075/570156 at 0.5 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plots showing the correlated expression of IL-17A in resting Left Plot or stimulated EL-4 cells Right Plot versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact EL-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of IL-17A expression in resting and stimulated Mouse EL-4 cells. Cells from the Mouse EL4 T lymphoma, ATCC TIB-39 cell line were either not stimulated Left Plot or stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724; Right Plot . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV805 Rat Anti-Mouse IL-17A antibody Cat. No. 570075/570156 at 0.5 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plots showing the correlated expression of IL-17A in resting Left Plot or stimulated EL-4 cells Right Plot versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact EL-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of IL-17A expression in resting and stimulated Mouse EL-4 cells. Cells from the Mouse EL4 T lymphoma, ATCC TIB-39 cell line were either not stimulated Left Plot or stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724; Right Plot . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV805 Rat Anti-Mouse IL-17A antibody Cat. No. 570075/570156 at 0.5 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plots showing the correlated expression of IL-17A in resting Left Plot or stimulated EL-4 cells Right Plot versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact EL-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of IL-17A expression in resting and stimulated Mouse EL-4 cells. Cells from the Mouse EL4 T lymphoma, ATCC TIB-39 cell line were either not stimulated Left Plot or stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724; Right Plot . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV805 Rat Anti-Mouse IL-17A antibody Cat. No. 570075/570156 at 0.5 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plots showing the correlated expression of IL-17A in resting Left Plot or stimulated EL-4 cells Right Plot versus cellular autofluorescence were derived from gated events with the forward and side light-scatter characteristics of intact EL-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.