BD HorizonTM BV421 Annexin V

Description

Staining cells with BD Horizon BV421 Annexin V and flow cytometric analysis of Jurkat cells undergoing apoptosis. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were cultured for 4 hours either alone Untreated Cells; dashed line histogram or with 6 muM camptothecin Treated Cells; solid line histogram . Cells were then harvested and stained with BD Horizon BV421 Annexin V Cat. No. 563973 and analyzed by flow cytometry. Untreated Cells were primarily BD Horizon BV421 Annexin V negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour culture with camptothecin, there were two populations of cells detected amongst Treated Cells: cells undergoing apoptosis BD Horizon BV421 Annexin V positive , and cells that were viable and not undergoing apoptosis BD Horizon BV421 Annexin V negative . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Staining cells with BD Horizon BV421 Annexin V and flow cytometric analysis of Jurkat cells undergoing apoptosis. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were cultured for 4 hours either alone Untreated Cells; dashed line histogram or with 6 muM camptothecin Treated Cells; solid line histogram . Cells were then harvested and stained with BD Horizon BV421 Annexin V Cat. No. 563973 and analyzed by flow cytometry. Untreated Cells were primarily BD Horizon BV421 Annexin V negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour culture with camptothecin, there were two populations of cells detected amongst Treated Cells: cells undergoing apoptosis BD Horizon BV421 Annexin V positive , and cells that were viable and not undergoing apoptosis BD Horizon BV421 Annexin V negative . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Staining cells with BD Horizon BV421 Annexin V and flow cytometric analysis of Jurkat cells undergoing apoptosis. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were cultured for 4 hours either alone Untreated Cells; dashed line histogram or with 6 muM camptothecin Treated Cells; solid line histogram . Cells were then harvested and stained with BD Horizon BV421 Annexin V Cat. No. 563973 and analyzed by flow cytometry. Untreated Cells were primarily BD Horizon BV421 Annexin V negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour culture with camptothecin, there were two populations of cells detected amongst Treated Cells: cells undergoing apoptosis BD Horizon BV421 Annexin V positive , and cells that were viable and not undergoing apoptosis BD Horizon BV421 Annexin V negative . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.