BD HorizonTM BV421 Armenian Hamster Anti-Mouse CD11c

Description

Flow cytometric analysis of CD11c expression on C57BL/6 mouse splenic leucocyte subsets. Panel 1. Splenic Leucocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E Cat. No. 562367 and either BD Horizon BV421 Hamster IgG2, lambda Isotype Control Cat. No. 562629; Left Plot or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody Cat. No. 565451/565452; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of I-A/I-E versus CD11c or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Panel 2. Ly6G/Ly6C-negative Splenic Leucocytes. Mouse splenic leucocytes were similarly preincubated with Mouse BD Fc Block and stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and PerCP-Cy5.5 Rat Anti-Mouse Ly6G/Ly6C Cat. No. 552093 antibodies and either BD Horizon BV421 Hamster IgG2, lambda Isotype Control Left Plot or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD45R/B220 versus CD11c or Ig Isotype control staining were derived from Ly6G/Ly6C- events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer system. Flow cytometric analysis of CD11c expression on C57BL/6 mouse splenic leucocyte subsets. Panel 1. Splenic Leucocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E Cat. No. 562367 and either BD Horizon BV421 Hamster IgG2, lambda Isotype Control Cat. No. 562629; Left Plot or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody Cat. No. 565451/565452; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of I-A/I-E versus CD11c or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Panel 2. Ly6G/Ly6C-negative Splenic Leucocytes. Mouse splenic leucocytes were similarly preincubated with Mouse BD Fc Block and stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and PerCP-Cy5.5 Rat Anti-Mouse Ly6G/Ly6C Cat. No. 552093 antibodies and either BD Horizon BV421 Hamster IgG2, lambda Isotype Control Left Plot or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD45R/B220 versus CD11c or Ig Isotype control staining were derived from Ly6G/Ly6C- events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer system. Show More Flow cytometric analysis of CD11c expression on C57BL/6 mouse splenic leucocyte subsets. Panel 1. Splenic Leucocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse I-A/I-E Cat. No. 562367 and either BD Horizon BV421 Hamster IgG2, lambda Isotype Control Cat. No. 562629; Left Plot or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody Cat. No. 565451/565452; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of I-A/I-E versus CD11c or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Panel 2. Ly6G/Ly6C-negative Splenic Leucocytes. Mouse splenic leucocytes were similarly preincubated with Mouse BD Fc Block and stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092/561880 and PerCP-Cy5.5 Rat Anti-Mouse Ly6G/Ly6C Cat. No. 552093 antibodies and either BD Horizon BV421 Hamster IgG2, lambda Isotype Control Left Plot or BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD45R/B220 versus CD11c or Ig Isotype control staining were derived from Ly6G/Ly6C- events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer system.