BD HorizonTM BV421 Goat Anti-Rabbit IgG

Description

Immunofluorescent Staining using BD Horizon BV421 Goat Anti-Rabbit Ig Left Panel - Immunofluorescence staining of apoptotic cells. HeLa cells were treated with Camptothecin 20 muM, 6 hr to induce apoptosis. Cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1 PBS, and blocked 30 min with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. The cells were stained 1 hr with Purified Mouse Anti-Cytochrome c Cat. No. 556432 and Purified Rabbit Anti-Active Caspase-3 Cat. No. 559565 antibodies at 2.5 mug/mL diluted in blocking buffer. After washing, the cells were stained 45 min with the second step reagents, BD Horizon BV421 Goat Anti-Rabbit Ig Cat. No. 565014; pseudo-colored green and BD Horizon BV480 Goat Anti-Mouse Ig Cat. No. 564877, pseudo-colored red antibodies at 2.5 mug/mL diluted in blocking buffer. DRAQ5 Cat. No. 564902/564903; pseudo-colored blue was used as a nuclear counterstain. Images were acquired with a standard epifluorescence microscope with a 20 objective. Right Panel - Multiparameter flow cytometric analysis of CD88 expression on human peripheral blood leucocytes. Whole blood was either not stained with a primary antibody as a control Top Plot or primarily stained with Purified Rabbit Anti-Human CD88 monoclonal antibody Cat. No. 559159; Bottom Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The cells were washed and secondarily stained with BD Horizon BV421 Goat Anti-Rabbit Ig Cat. No. 565014 . Two-parameter flow cytometric contour plots showing the correlated expression of CD88 or Unstained control versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Immunofluorescent Staining using BD Horizon BV421 Goat Anti-Rabbit Ig Left Panel - Immunofluorescence staining of apoptotic cells. HeLa cells were treated with Camptothecin 20 muM, 6 hr to induce apoptosis. Cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1 PBS, and blocked 30 min with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. The cells were stained 1 hr with Purified Mouse Anti-Cytochrome c Cat. No. 556432 and Purified Rabbit Anti-Active Caspase-3 Cat. No. 559565 antibodies at 2.5 mug/mL diluted in blocking buffer. After washing, the cells were stained 45 min with the second step reagents, BD Horizon BV421 Goat Anti-Rabbit Ig Cat. No. 565014; pseudo-colored green and BD Horizon BV480 Goat Anti-Mouse Ig Cat. No. 564877, pseudo-colored red antibodies at 2.5 mug/mL diluted in blocking buffer. DRAQ5 Cat. No. 564902/564903; pseudo-colored blue was used as a nuclear counterstain. Images were acquired with a standard epifluorescence microscope with a 20 objective. Right Panel - Multiparameter flow cytometric analysis of CD88 expression on human peripheral blood leucocytes. Whole blood was either not stained with a primary antibody as a control Top Plot or primarily stained with Purified Rabbit Anti-Human CD88 monoclonal antibody Cat. No. 559159; Bottom Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The cells were washed and secondarily stained with BD Horizon BV421 Goat Anti-Rabbit Ig Cat. No. 565014 . Two-parameter flow cytometric contour plots showing the correlated expression of CD88 or Unstained control versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Immunofluorescent Staining using BD Horizon BV421 Goat Anti-Rabbit Ig Left Panel - Immunofluorescence staining of apoptotic cells. HeLa cells were treated with Camptothecin 20 muM, 6 hr to induce apoptosis. Cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1 PBS, and blocked 30 min with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1 PBS. The cells were stained 1 hr with Purified Mouse Anti-Cytochrome c Cat. No. 556432 and Purified Rabbit Anti-Active Caspase-3 Cat. No. 559565 antibodies at 2.5 mug/mL diluted in blocking buffer. After washing, the cells were stained 45 min with the second step reagents, BD Horizon BV421 Goat Anti-Rabbit Ig Cat. No. 565014; pseudo-colored green and BD Horizon BV480 Goat Anti-Mouse Ig Cat. No. 564877, pseudo-colored red antibodies at 2.5 mug/mL diluted in blocking buffer. DRAQ5 Cat. No. 564902/564903; pseudo-colored blue was used as a nuclear counterstain. Images were acquired with a standard epifluorescence microscope with a 20 objective. Right Panel - Multiparameter flow cytometric analysis of CD88 expression on human peripheral blood leucocytes. Whole blood was either not stained with a primary antibody as a control Top Plot or primarily stained with Purified Rabbit Anti-Human CD88 monoclonal antibody Cat. No. 559159; Bottom Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The cells were washed and secondarily stained with BD Horizon BV421 Goat Anti-Rabbit Ig Cat. No. 565014 . Two-parameter flow cytometric contour plots showing the correlated expression of CD88 or Unstained control versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.