
566285 · 25 ug
BDB Bioscience · Cat: 566285
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD80 on resting or stimulated mouse splenocytes. Fresh isolated Left Panel or 72-hour LPS-stimulated C57BL/6 splenocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and stained either with a BD Horizon BV421 Hamster IgG2, kappa isotype control Cat. No. 562612; dashed line histogram or with the BD Horizon BV421 Hamster Anti-Mouse CD80 antibody Cat. No. 562611; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD80 on resting or stimulated mouse splenocytes. Fresh isolated Left Panel or 72-hour LPS-stimulated C57BL/6 splenocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and stained either with a BD Horizon BV421 Hamster IgG2, kappa isotype control Cat. No. 562612; dashed line histogram or with the BD Horizon BV421 Hamster Anti-Mouse CD80 antibody Cat. No. 562611; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD80 on resting or stimulated mouse splenocytes. Fresh isolated Left Panel or 72-hour LPS-stimulated C57BL/6 splenocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and stained either with a BD Horizon BV421 Hamster IgG2, kappa isotype control Cat. No. 562612; dashed line histogram or with the BD Horizon BV421 Hamster Anti-Mouse CD80 antibody Cat. No. 562611; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD80 on resting or stimulated mouse splenocytes. Fresh isolated Left Panel or 72-hour LPS-stimulated C57BL/6 splenocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and stained either with a BD Horizon BV421 Hamster IgG2, kappa isotype control Cat. No. 562612; dashed line histogram or with the BD Horizon BV421 Hamster Anti-Mouse CD80 antibody Cat. No. 562611; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD80 on resting or stimulated mouse splenocytes. Fresh isolated Left Panel or 72-hour LPS-stimulated C57BL/6 splenocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and stained either with a BD Horizon BV421 Hamster IgG2, kappa isotype control Cat. No. 562612; dashed line histogram or with the BD Horizon BV421 Hamster Anti-Mouse CD80 antibody Cat. No. 562611; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD80 on resting or stimulated mouse splenocytes. Fresh isolated Left Panel or 72-hour LPS-stimulated C57BL/6 splenocytes Right Panel were pretreated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and stained either with a BD Horizon BV421 Hamster IgG2, kappa isotype control Cat. No. 562612; dashed line histogram or with the BD Horizon BV421 Hamster Anti-Mouse CD80 antibody Cat. No. 562611; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.