BD HorizonTM BV421 Hamster Anti-Mouse CXCL9 (MIG)

Description

Flow cytometric analysis of CXCL9 MIG expression in Unstimulated and Stimulated Mouse RAW264.7 cells. RAW 264.7 Macrophage; ATCC TIB-71 cells were cultured 2 hours without Unstimulated; dashed line histogram or with Stimulated; solid line histogram Recombinant Mouse IFN-gamma protein Cat. No. 554587; 10 ng/ml followed by LPS 1 mug/ml . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was added for overnight culture. Cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Hamster Anti-Mouse CXCL9 MIG antibody Cat. No. 570803/570879 at 0.5 mug/test. The histograms showing CXCL9 MIG expression in Unstimulated versus Stimulated cells were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CXCL9 MIG expression in Unstimulated and Stimulated Mouse RAW264.7 cells. RAW 264.7 Macrophage; ATCC TIB-71 cells were cultured 2 hours without Unstimulated; dashed line histogram or with Stimulated; solid line histogram Recombinant Mouse IFN-gamma protein Cat. No. 554587; 10 ng/ml followed by LPS 1 mug/ml . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was added for overnight culture. Cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Hamster Anti-Mouse CXCL9 MIG antibody Cat. No. 570803/570879 at 0.5 mug/test. The histograms showing CXCL9 MIG expression in Unstimulated versus Stimulated cells were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CXCL9 MIG expression in Unstimulated and Stimulated Mouse RAW264.7 cells. RAW 264.7 Macrophage; ATCC TIB-71 cells were cultured 2 hours without Unstimulated; dashed line histogram or with Stimulated; solid line histogram Recombinant Mouse IFN-gamma protein Cat. No. 554587; 10 ng/ml followed by LPS 1 mug/ml . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was added for overnight culture. Cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Hamster Anti-Mouse CXCL9 MIG antibody Cat. No. 570803/570879 at 0.5 mug/test. The histograms showing CXCL9 MIG expression in Unstimulated versus Stimulated cells were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.