BD HorizonTM BV421 Mouse Anti-Cleaved PARP (Asp 214)

Description

Flow cytometric analysis of cleaved PARP Asp 214 expression left panel . Cells from the human Jurkat ATCC TIB-152 cell line were cultured for 6 hours with solid line histogram or without dashed line histogram Camptothecin Sigma-Aldrich Cat. No. C-9911; 12 muM final concentration . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Cleaved PARP Asp 214 antibody Cat. No. 564129 . Histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunofluorescent analysis of cleaved PARP in apoptotic human cells right panel . HeLa cells ATCC, CCL-2 were treated with camptothecin 20 muM, 6 hours to induce apoptosis. Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Cleaved PARP Asp 214 antibody Cat. No. 564129, pseudo-colored red and Alexa Fluor 488 Mouse Anti-GM130 antibody Cat. No. 560257, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of cleaved PARP Asp 214 expression left panel . Cells from the human Jurkat ATCC TIB-152 cell line were cultured for 6 hours with solid line histogram or without dashed line histogram Camptothecin Sigma-Aldrich Cat. No. C-9911; 12 muM final concentration . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Cleaved PARP Asp 214 antibody Cat. No. 564129 . Histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunofluorescent analysis of cleaved PARP in apoptotic human cells right panel . HeLa cells ATCC, CCL-2 were treated with camptothecin 20 muM, 6 hours to induce apoptosis. Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Cleaved PARP Asp 214 antibody Cat. No. 564129, pseudo-colored red and Alexa Fluor 488 Mouse Anti-GM130 antibody Cat. No. 560257, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Flow cytometric analysis of cleaved PARP Asp 214 expression left panel . Cells from the human Jurkat ATCC TIB-152 cell line were cultured for 6 hours with solid line histogram or without dashed line histogram Camptothecin Sigma-Aldrich Cat. No. C-9911; 12 muM final concentration . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Cleaved PARP Asp 214 antibody Cat. No. 564129 . Histograms were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunofluorescent analysis of cleaved PARP in apoptotic human cells right panel . HeLa cells ATCC, CCL-2 were treated with camptothecin 20 muM, 6 hours to induce apoptosis. Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-Cleaved PARP Asp 214 antibody Cat. No. 564129, pseudo-colored red and Alexa Fluor 488 Mouse Anti-GM130 antibody Cat. No. 560257, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.