BD HorizonTM BV421 Mouse Anti-CXCR7

Description

Flow cytometric analysis of CXCR7 expression on mouse leukocytes Left Panel . C57BL/6 splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block, Cat. No. 553141 or 553142 . To demonstrate the expression of CXCR7 on marginal-zone B lymphocytes, the splenocytes were co-stained with six fluorochrome-conjugated monoclonal antibodies in the BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349/566385 : FITC Rat Anti-Mouse CD21/CD35 Cat. No. 553818 or 561769 , Alexa Fluor 647 Rat Anti-Mouse CD9 Cat. No. 564233 , BD Horizon PE-CF594 Rat Anti-Mouse CD45R Cat. No. 562290 or 562313 , BD Horizon BUV737 Rat Anti-Mouse CD23 Cat. No. 564436 , BD Horizon BUV395 Rat Anti-Mouse IgM Cat. No. 564025 and either BD Horizon BV421 Mouse Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439; dotted-line histograms . The histograms were derived from gated viable splenocytes expressing with the B220-positive, IgM-high, CD21-high, CD23-low, CD9-positive phenotype. Flow cytometric analysis of CXCR7 expression on human CXCR7-transfected 293F cells Middle and Right Panels . Untransfected 293F cells Middle Panel and 293F cells transfected with a human CXCR7 construct Right Panel were initially fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then stained with either BD Horizon BV421 Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439, dotted-line histogram . The histograms were derived from gated events based on the light scattering characteristics of intact 293F cells. Flow cytometry was performed on a BD LSRFortessa X-20 Cell Analyzer. Flow cytometric analysis of CXCR7 expression on mouse leukocytes Left Panel . C57BL/6 splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block, Cat. No. 553141 or 553142 . To demonstrate the expression of CXCR7 on marginal-zone B lymphocytes, the splenocytes were co-stained with six fluorochrome-conjugated monoclonal antibodies in the BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349/566385 : FITC Rat Anti-Mouse CD21/CD35 Cat. No. 553818 or 561769 , Alexa Fluor 647 Rat Anti-Mouse CD9 Cat. No. 564233 , BD Horizon PE-CF594 Rat Anti-Mouse CD45R Cat. No. 562290 or 562313 , BD Horizon BUV737 Rat Anti-Mouse CD23 Cat. No. 564436 , BD Horizon BUV395 Rat Anti-Mouse IgM Cat. No. 564025 and either BD Horizon BV421 Mouse Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439; dotted-line histograms . The histograms were derived from gated viable splenocytes expressing with the B220-positive, IgM-high, CD21-high, CD23-low, CD9-positive phenotype. Flow cytometric analysis of CXCR7 expression on human CXCR7-transfected 293F cells Middle and Right Panels . Untransfected 293F cells Middle Panel and 293F cells transfected with a human CXCR7 construct Right Panel were initially fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then stained with either BD Horizon BV421 Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439, dotted-line histogram . The histograms were derived from gated events based on the light scattering characteristics of intact 293F cells. Flow cytometry was performed on a BD LSRFortessa X-20 Cell Analyzer. Flow cytometric analysis of CXCR7 expression on mouse leukocytes Left Panel . C57BL/6 splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block, Cat. No. 553141 or 553142 . To demonstrate the expression of CXCR7 on marginal-zone B lymphocytes, the splenocytes were co-stained with six fluorochrome-conjugated monoclonal antibodies in the BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349/566385 : FITC Rat Anti-Mouse CD21/CD35 Cat. No. 553818 or 561769 , Alexa Fluor 647 Rat Anti-Mouse CD9 Cat. No. 564233 , BD Horizon PE-CF594 Rat Anti-Mouse CD45R Cat. No. 562290 or 562313 , BD Horizon BUV737 Rat Anti-Mouse CD23 Cat. No. 564436 , BD Horizon BUV395 Rat Anti-Mouse IgM Cat. No. 564025 and either BD Horizon BV421 Mouse Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439; dotted-line histograms . The histograms were derived from gated viable splenocytes expressing with the B220-positive, IgM-high, CD21-high, CD23-low, CD9-positive phenotype. Flow cytometric analysis of CXCR7 expression on human CXCR7-transfected 293F cells Middle and Right Panels . Untransfected 293F cells Middle Panel and 293F cells transfected with a human CXCR7 construct Right Panel were initially fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then stained with either BD Horizon BV421 Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439, dotted-line histogram . The histograms were derived from gated events based on the light scattering characteristics of intact 293F cells. Flow cytometry was performed on a BD LSRFortessa X-20 Cell Analyzer. Show More Flow cytometric analysis of CXCR7 expression on mouse leukocytes Left Panel . C57BL/6 splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block, Cat. No. 553141 or 553142 . To demonstrate the expression of CXCR7 on marginal-zone B lymphocytes, the splenocytes were co-stained with six fluorochrome-conjugated monoclonal antibodies in the BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349/566385 : FITC Rat Anti-Mouse CD21/CD35 Cat. No. 553818 or 561769 , Alexa Fluor 647 Rat Anti-Mouse CD9 Cat. No. 564233 , BD Horizon PE-CF594 Rat Anti-Mouse CD45R Cat. No. 562290 or 562313 , BD Horizon BUV737 Rat Anti-Mouse CD23 Cat. No. 564436 , BD Horizon BUV395 Rat Anti-Mouse IgM Cat. No. 564025 and either BD Horizon BV421 Mouse Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439; dotted-line histograms . The histograms were derived from gated viable splenocytes expressing with the B220-positive, IgM-high, CD21-high, CD23-low, CD9-positive phenotype. Flow cytometric analysis of CXCR7 expression on human CXCR7-transfected 293F cells Middle and Right Panels . Untransfected 293F cells Middle Panel and 293F cells transfected with a human CXCR7 construct Right Panel were initially fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then stained with either BD Horizon BV421 Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439, dotted-line histogram . The histograms were derived from gated events based on the light scattering characteristics of intact 293F cells. Flow cytometry was performed on a BD LSRFortessa X-20 Cell Analyzer. Show More Flow cytometric analysis of CXCR7 expression on mouse leukocytes Left Panel . C57BL/6 splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block, Cat. No. 553141 or 553142 . To demonstrate the expression of CXCR7 on marginal-zone B lymphocytes, the splenocytes were co-stained with six fluorochrome-conjugated monoclonal antibodies in the BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349/566385 : FITC Rat Anti-Mouse CD21/CD35 Cat. No. 553818 or 561769 , Alexa Fluor 647 Rat Anti-Mouse CD9 Cat. No. 564233 , BD Horizon PE-CF594 Rat Anti-Mouse CD45R Cat. No. 562290 or 562313 , BD Horizon BUV737 Rat Anti-Mouse CD23 Cat. No. 564436 , BD Horizon BUV395 Rat Anti-Mouse IgM Cat. No. 564025 and either BD Horizon BV421 Mouse Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439; dotted-line histograms . The histograms were derived from gated viable splenocytes expressing with the B220-positive, IgM-high, CD21-high, CD23-low, CD9-positive phenotype. Flow cytometric analysis of CXCR7 expression on human CXCR7-transfected 293F cells Middle and Right Panels . Untransfected 293F cells Middle Panel and 293F cells transfected with a human CXCR7 construct Right Panel were initially fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then stained with either BD Horizon BV421 Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439, dotted-line histogram . The histograms were derived from gated events based on the light scattering characteristics of intact 293F cells. Flow cytometry was performed on a BD LSRFortessa X-20 Cell Analyzer. Flow cytometric analysis of CXCR7 expression on mouse leukocytes Left Panel . C57BL/6 splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block, Cat. No. 553141 or 553142 . To demonstrate the expression of CXCR7 on marginal-zone B lymphocytes, the splenocytes were co-stained with six fluorochrome-conjugated monoclonal antibodies in the BD Horizon Brilliant Stain Buffer Cat. No. 563794/566349/566385 : FITC Rat Anti-Mouse CD21/CD35 Cat. No. 553818 or 561769 , Alexa Fluor 647 Rat Anti-Mouse CD9 Cat. No. 564233 , BD Horizon PE-CF594 Rat Anti-Mouse CD45R Cat. No. 562290 or 562313 , BD Horizon BUV737 Rat Anti-Mouse CD23 Cat. No. 564436 , BD Horizon BUV395 Rat Anti-Mouse IgM Cat. No. 564025 and either BD Horizon BV421 Mouse Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439; dotted-line histograms . The histograms were derived from gated viable splenocytes expressing with the B220-positive, IgM-high, CD21-high, CD23-low, CD9-positive phenotype. Flow cytometric analysis of CXCR7 expression on human CXCR7-transfected 293F cells Middle and Right Panels . Untransfected 293F cells Middle Panel and 293F cells transfected with a human CXCR7 construct Right Panel were initially fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then stained with either BD Horizon BV421 Anti-CXCR7 Cat. No. 566233 or 566234, solid-line histogram or BD Horizon BV421 Mouse IgG2a, k Isotype Control Cat. No. 562439, dotted-line histogram . The histograms were derived from gated events based on the light scattering characteristics of intact 293F cells. Flow cytometry was performed on a BD LSRFortessa X-20 Cell Analyzer.