
567166 ·
BDB Bioscience · Cat: 567166
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of EOMES expression in human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stained intracellularly with PE Mouse Anti-Human CD8 antibody Cat. No. 555367/557086/561949 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-EOMES antibody Cat. No. 567166; Right Plot at 0.5 mug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The bivariate pseudocolor density plot showing the correlated expression of EOMES or Ig Isotype control staining versus CD8 was derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EOMES expression in mouse splenic leucocytes. Spleen cells from C57BL/6 mice were stained intracellularly with PE Rat Anti-Mouse CD335 NKp46 antibody Cat.No. 560757 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BV421 Mouse Anti-EOMES antibody Right Plot at 0.5 mug/test using BD Pharmingen Transcription Factor Buffer Set. The bivariate pseudocolor density plot showing the correlated expression of EOMES or Ig isotype control staining versus CD355 NKp46 were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EOMES expression in human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stained intracellularly with PE Mouse Anti-Human CD8 antibody Cat. No. 555367/557086/561949 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-EOMES antibody Cat. No. 567166; Right Plot at 0.5 mug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The bivariate pseudocolor density plot showing the correlated expression of EOMES or Ig Isotype control staining versus CD8 was derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of EOMES expression in mouse splenic leucocytes. Spleen cells from C57BL/6 mice were stained intracellularly with PE Rat Anti-Mouse CD335 NKp46 antibody Cat.No. 560757 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BV421 Mouse Anti-EOMES antibody Right Plot at 0.5 mug/test using BD Pharmingen Transcription Factor Buffer Set. The bivariate pseudocolor density plot showing the correlated expression of EOMES or Ig isotype control staining versus CD355 NKp46 were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of EOMES expression in human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stained intracellularly with PE Mouse Anti-Human CD8 antibody Cat. No. 555367/557086/561949 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-EOMES antibody Cat. No. 567166; Right Plot at 0.5 mug/test using BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The bivariate pseudocolor density plot showing the correlated expression of EOMES or Ig Isotype control staining versus CD8 was derived from events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EOMES expression in mouse splenic leucocytes. Spleen cells from C57BL/6 mice were stained intracellularly with PE Rat Anti-Mouse CD335 NKp46 antibody Cat.No. 560757 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BV421 Mouse Anti-EOMES antibody Right Plot at 0.5 mug/test using BD Pharmingen Transcription Factor Buffer Set. The bivariate pseudocolor density plot showing the correlated expression of EOMES or Ig isotype control staining versus CD355 NKp46 were derived from events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.