BD HorizonTM BV421 Mouse Anti-EZH2

Description

Flow cytometric analysis of EZH2 expression in Jurkat cells left . Cells from the human Jurkat T-cell leukemia cell line ATCC TIB-152 were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and then stained with matching concentrations of either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse anti-EZH2 monoclonal antibody Cat. No. 562963; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunofluorescent analysis of EZH2 expression by human cells right . Cultured cells from the HeLa cell line ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-EZH2 antibody Cat. No. 562963, pseudo-colored red and Alexa Fluor 488 Mouse Anti-Cytochrome c Cat. No. 560263, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard four laser confocal microscope. Original magnification, 40x. Flow cytometric analysis of EZH2 expression in Jurkat cells left . Cells from the human Jurkat T-cell leukemia cell line ATCC TIB-152 were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and then stained with matching concentrations of either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse anti-EZH2 monoclonal antibody Cat. No. 562963; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunofluorescent analysis of EZH2 expression by human cells right . Cultured cells from the HeLa cell line ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-EZH2 antibody Cat. No. 562963, pseudo-colored red and Alexa Fluor 488 Mouse Anti-Cytochrome c Cat. No. 560263, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard four laser confocal microscope. Original magnification, 40x. Show More Flow cytometric analysis of EZH2 expression in Jurkat cells left . Cells from the human Jurkat T-cell leukemia cell line ATCC TIB-152 were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and then stained with matching concentrations of either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse anti-EZH2 monoclonal antibody Cat. No. 562963; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Immunofluorescent analysis of EZH2 expression by human cells right . Cultured cells from the HeLa cell line ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-EZH2 antibody Cat. No. 562963, pseudo-colored red and Alexa Fluor 488 Mouse Anti-Cytochrome c Cat. No. 560263, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard four laser confocal microscope. Original magnification, 40x.