BD HorizonTM BV421 Mouse Anti-GATA3

Description

Flow cytometric analysis of GATA3 expression left and center panels . Comparison of GATA3 expression in human T and B cell lines left panel . Cells from the Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram and Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV421 Mouse Anti-GATA3 antibody Cat. No. 563349 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Comparison of GATA3 expression in mouse Th1 and Th2 cells center panel . Cloned D10.G4.1 Th2 lymphoblasts ATCC TIB-224; solid line histogram and 2D6 Th1 cells dashed line histogram were similarly fixed, permeabilized, stained with BD Horizon BV421 Mouse Anti-GATA3 antibody and analyzed by flow cytometry. Immunofluorescent analysis of Gata-3 expression by human cells right panel . Cultured cells from the MCF7 cell line ATCC HTB-22 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-GATA3 antibody Cat. No. 563349, pseudo-colored red and Alexa Fluor 488 Mouse Anti-E-cadherin antibody Cat. No. 560061, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard four laser confocal microscope. Original magnification, 40x. Flow cytometric analysis of GATA3 expression left and center panels . Comparison of GATA3 expression in human T and B cell lines left panel . Cells from the Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram and Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV421 Mouse Anti-GATA3 antibody Cat. No. 563349 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Comparison of GATA3 expression in mouse Th1 and Th2 cells center panel . Cloned D10.G4.1 Th2 lymphoblasts ATCC TIB-224; solid line histogram and 2D6 Th1 cells dashed line histogram were similarly fixed, permeabilized, stained with BD Horizon BV421 Mouse Anti-GATA3 antibody and analyzed by flow cytometry. Immunofluorescent analysis of Gata-3 expression by human cells right panel . Cultured cells from the MCF7 cell line ATCC HTB-22 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-GATA3 antibody Cat. No. 563349, pseudo-colored red and Alexa Fluor 488 Mouse Anti-E-cadherin antibody Cat. No. 560061, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard four laser confocal microscope. Original magnification, 40x. Show More Flow cytometric analysis of GATA3 expression left and center panels . Comparison of GATA3 expression in human T and B cell lines left panel . Cells from the Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram and Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV421 Mouse Anti-GATA3 antibody Cat. No. 563349 . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Comparison of GATA3 expression in mouse Th1 and Th2 cells center panel . Cloned D10.G4.1 Th2 lymphoblasts ATCC TIB-224; solid line histogram and 2D6 Th1 cells dashed line histogram were similarly fixed, permeabilized, stained with BD Horizon BV421 Mouse Anti-GATA3 antibody and analyzed by flow cytometry. Immunofluorescent analysis of Gata-3 expression by human cells right panel . Cultured cells from the MCF7 cell line ATCC HTB-22 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and blocked with 5 % goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in PBS. Cells were stained with BD Horizon BV421 Mouse Anti-GATA3 antibody Cat. No. 563349, pseudo-colored red and Alexa Fluor 488 Mouse Anti-E-cadherin antibody Cat. No. 560061, pseudo-colored green . DRAQ5 was used as a nuclear counterstain Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard four laser confocal microscope. Original magnification, 40x.