BD HorizonTM BV421 Mouse Anti-Glucagon

Description

Flow cytometric analysis of Glucagon expression in a mouse pancreatic alpha cell line. Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either BD Horizon BV421 Mouse IgG1, k Isotype Control Cat. No. 562438, dotted-line histogram or BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, solid-line histogram . The fluorescence histogram was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Immunohistofluorescent staining of Glucagon in human, mouse, and rat islets of Langerhans. Following antigen retrieval with BD Pharmingen Retrievagen A Buffer Cat. No. 550524 , sections from formalin-fixed, paraffin-embedded human top row , mouse middle row , and rat bottom row pancreata were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. LEFT PANEL: These sections were then stained with BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, pseudocolored red , and cell nuclei were counterstained with BD Pharmingen DRAQ5 Cat. No. 564902 pseudo-colored green . RIGHT PANEL: These sections were then stained with BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, pseudocolored red and Alexa Fluor 647 Mouse Anti-Insulin Cat. No.565689, pseudocolored green . The cell nuclei were counterstained with BD Pharmingen 7-AAD Cat. No. 559925, pseudocolored blue, top and middle rows only . The typical localization of the alpha cells is seen: distributed throughout human islets and at the periphery in rodent islets. The photographs were performed on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of Glucagon expression in a mouse pancreatic alpha cell line. Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either BD Horizon BV421 Mouse IgG1, k Isotype Control Cat. No. 562438, dotted-line histogram or BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, solid-line histogram . The fluorescence histogram was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Immunohistofluorescent staining of Glucagon in human, mouse, and rat islets of Langerhans. Following antigen retrieval with BD Pharmingen Retrievagen A Buffer Cat. No. 550524 , sections from formalin-fixed, paraffin-embedded human top row , mouse middle row , and rat bottom row pancreata were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. LEFT PANEL: These sections were then stained with BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, pseudocolored red , and cell nuclei were counterstained with BD Pharmingen DRAQ5 Cat. No. 564902 pseudo-colored green . RIGHT PANEL: These sections were then stained with BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, pseudocolored red and Alexa Fluor 647 Mouse Anti-Insulin Cat. No.565689, pseudocolored green . The cell nuclei were counterstained with BD Pharmingen 7-AAD Cat. No. 559925, pseudocolored blue, top and middle rows only . The typical localization of the alpha cells is seen: distributed throughout human islets and at the periphery in rodent islets. The photographs were performed on a standard epifluorescence microscope. Original magnification, 20x. Show More Flow cytometric analysis of Glucagon expression in a mouse pancreatic alpha cell line. Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either BD Horizon BV421 Mouse IgG1, k Isotype Control Cat. No. 562438, dotted-line histogram or BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, solid-line histogram . The fluorescence histogram was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Immunohistofluorescent staining of Glucagon in human, mouse, and rat islets of Langerhans. Following antigen retrieval with BD Pharmingen Retrievagen A Buffer Cat. No. 550524 , sections from formalin-fixed, paraffin-embedded human top row , mouse middle row , and rat bottom row pancreata were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. LEFT PANEL: These sections were then stained with BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, pseudocolored red , and cell nuclei were counterstained with BD Pharmingen DRAQ5 Cat. No. 564902 pseudo-colored green . RIGHT PANEL: These sections were then stained with BD Horizon BV421 Mouse Anti-Glucagon Cat. No. 565891, pseudocolored red and Alexa Fluor 647 Mouse Anti-Insulin Cat. No.565689, pseudocolored green . The cell nuclei were counterstained with BD Pharmingen 7-AAD Cat. No. 559925, pseudocolored blue, top and middle rows only . The typical localization of the alpha cells is seen: distributed throughout human islets and at the periphery in rodent islets. The photographs were performed on a standard epifluorescence microscope. Original magnification, 20x.