BD HorizonTM BV421 Mouse Anti-Human CCR7 (CD197)

Description

Flow cytometric analysis of CCR7 CD197 on human peripheral blood lymphocytes. Whole blood was stained with Alexa Fluor647 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 557707 , PE Mouse Anti-Human CD45RA clone HI100, Cat. No. 555489 or 561883 , and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CCR7 CD197 Cat. No. 566743 or 566744, Right Plot . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Two-color flow cytometric contour plots showing the correlated patterns of CD45RA versus CCR7 CD197 or Ig isotype control staining were derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunohistofluorescent staining of CCR7 CD197 in human tonsil. The section of acetone-fixed frozen tissue was stained with BD Horizon BV421 Mouse Anti-Human CCR7 CD197 Cat. No. 566743 or 566744, pseudocolored green , and cell nuclei were counterstained with DRAQ5 Cat. No. 564902 or 564903, pseudocolored red . The CCR7 CD197 staining identifies T lymphocytes among the lymphoid follicles. Photography was performed on a standard epifluorescence microscope. Original magnification, 20. Flow cytometric analysis of CCR7 CD197 on human peripheral blood lymphocytes. Whole blood was stained with Alexa Fluor647 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 557707 , PE Mouse Anti-Human CD45RA clone HI100, Cat. No. 555489 or 561883 , and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CCR7 CD197 Cat. No. 566743 or 566744, Right Plot . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Two-color flow cytometric contour plots showing the correlated patterns of CD45RA versus CCR7 CD197 or Ig isotype control staining were derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Immunohistofluorescent staining of CCR7 CD197 in human tonsil. The section of acetone-fixed frozen tissue was stained with BD Horizon BV421 Mouse Anti-Human CCR7 CD197 Cat. No. 566743 or 566744, pseudocolored green , and cell nuclei were counterstained with DRAQ5 Cat. No. 564902 or 564903, pseudocolored red . The CCR7 CD197 staining identifies T lymphocytes among the lymphoid follicles. Photography was performed on a standard epifluorescence microscope. Original magnification, 20. Show More Flow cytometric analysis of CCR7 CD197 on human peripheral blood lymphocytes. Whole blood was stained with Alexa Fluor647 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 557707 , PE Mouse Anti-Human CD45RA clone HI100, Cat. No. 555489 or 561883 , and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CCR7 CD197 Cat. No. 566743 or 566744, Right Plot . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Two-color flow cytometric contour plots showing the correlated patterns of CD45RA versus CCR7 CD197 or Ig isotype control staining were derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunohistofluorescent staining of CCR7 CD197 in human tonsil. The section of acetone-fixed frozen tissue was stained with BD Horizon BV421 Mouse Anti-Human CCR7 CD197 Cat. No. 566743 or 566744, pseudocolored green , and cell nuclei were counterstained with DRAQ5 Cat. No. 564902 or 564903, pseudocolored red . The CCR7 CD197 staining identifies T lymphocytes among the lymphoid follicles. Photography was performed on a standard epifluorescence microscope. Original magnification, 20.