BD HorizonTM BV421 Mouse Anti-Human CD11c

Description

Analysis of CD11c Expression Flow cytometric analysis of CD11c expression on Human Panel 1a or Rhesus macaque Panel 1b peripheral blood leucocytes. Human or Rhesus macaque whole blood collected with heparin as the preferred anticoagulant rather than EDTA was stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plots or BD Horizon BV421 Mouse Anti-Human CD11c antibody Cat. No. 565806/565807; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric contour plots showing the correlated expression of CD11c or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Routine flow cytometric analysis is performed on human peripheral blood leucocytes. Data shown on this Technical Data Sheet are not lot specific. Three-color Immunofluorescence IF analysis of CD11c expression by cells in human tonsil Panel 2 . A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with Purified Mouse Anti-Human CD3 antibody Cat. No. 555330 followed by BD Horizon BV480 Goat Anti-Mouse Ig second step antibody Cat. No. 564877, pseudocolored red . Sections were thoroughly washed, then stained with BD Horizon BV421 Mouse Anti-Human CD11c antibody Cat. No. 565806/565807, pseudocolored green and Alexa Fluor 488 CD19 antibody Cat. No. 557697, pseudocolored blue . Slides were mounted with ProLong Gold. The images were captured using a standard epifluorescence microscope and merged. Original magnification, 20 . Analysis of CD11c Expression Flow cytometric analysis of CD11c expression on Human Panel 1a or Rhesus macaque Panel 1b peripheral blood leucocytes. Human or Rhesus macaque whole blood collected with heparin as the preferred anticoagulant rather than EDTA was stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plots or BD Horizon BV421 Mouse Anti-Human CD11c antibody Cat. No. 565806/565807; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric contour plots showing the correlated expression of CD11c or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Routine flow cytometric analysis is performed on human peripheral blood leucocytes. Data shown on this Technical Data Sheet are not lot specific. Three-color Immunofluorescence IF analysis of CD11c expression by cells in human tonsil Panel 2 . A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with Purified Mouse Anti-Human CD3 antibody Cat. No. 555330 followed by BD Horizon BV480 Goat Anti-Mouse Ig second step antibody Cat. No. 564877, pseudocolored red . Sections were thoroughly washed, then stained with BD Horizon BV421 Mouse Anti-Human CD11c antibody Cat. No. 565806/565807, pseudocolored green and Alexa Fluor 488 CD19 antibody Cat. No. 557697, pseudocolored blue . Slides were mounted with ProLong Gold. The images were captured using a standard epifluorescence microscope and merged. Original magnification, 20 . Show More Analysis of CD11c Expression Flow cytometric analysis of CD11c expression on Human Panel 1a or Rhesus macaque Panel 1b peripheral blood leucocytes. Human or Rhesus macaque whole blood collected with heparin as the preferred anticoagulant rather than EDTA was stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plots or BD Horizon BV421 Mouse Anti-Human CD11c antibody Cat. No. 565806/565807; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric contour plots showing the correlated expression of CD11c or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Routine flow cytometric analysis is performed on human peripheral blood leucocytes. Data shown on this Technical Data Sheet are not lot specific. Three-color Immunofluorescence IF analysis of CD11c expression by cells in human tonsil Panel 2 . A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with Purified Mouse Anti-Human CD3 antibody Cat. No. 555330 followed by BD Horizon BV480 Goat Anti-Mouse Ig second step antibody Cat. No. 564877, pseudocolored red . Sections were thoroughly washed, then stained with BD Horizon BV421 Mouse Anti-Human CD11c antibody Cat. No. 565806/565807, pseudocolored green and Alexa Fluor 488 CD19 antibody Cat. No. 557697, pseudocolored blue . Slides were mounted with ProLong Gold. The images were captured using a standard epifluorescence microscope and merged. Original magnification, 20 .