BD HorizonTM BV421 Mouse Anti-Human CD154

Description

Flow cytometric analysis of CD154 expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD154 antibody Cat. No. 563886; solid line histogram . The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD154 expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD154 antibody Cat. No. 563886; solid line histogram . The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD154 expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD154 antibody Cat. No. 563886; solid line histogram . The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.