BD HorizonTM BV421 Mouse Anti-Human CD19

Description

Immunohistofluorescent analysis of CD19 expression by cells within human tonsil. A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441, pseudo-colored green , Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695, pseudo-colored red , and Alexa Fluor 555 Mouse Anti-Human Ki-67 antibody Cat. No. 558617, pseudo-colored yellow . The section was then counterstained with DRAQ5 Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of CD19 expression on human peripheral blood lymphocytes . Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441; solid line histogram or with BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Immunohistofluorescent analysis of CD19 expression by cells within human tonsil. A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441, pseudo-colored green , Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695, pseudo-colored red , and Alexa Fluor 555 Mouse Anti-Human Ki-67 antibody Cat. No. 558617, pseudo-colored yellow . The section was then counterstained with DRAQ5 Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Flow cytometric analysis of CD19 expression on human peripheral blood lymphocytes . Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441; solid line histogram or with BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Show More Immunohistofluorescent analysis of CD19 expression by cells within human tonsil. A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441, pseudo-colored green , Alexa Fluor 488 Mouse Anti-Human CD4 antibody Cat. No. 557695, pseudo-colored red , and Alexa Fluor 555 Mouse Anti-Human Ki-67 antibody Cat. No. 558617, pseudo-colored yellow . The section was then counterstained with DRAQ5 Cat. No. 564902/564903, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Flow cytometric analysis of CD19 expression on human peripheral blood lymphocytes . Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441; solid line histogram or with BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System.