
567788 · 25 Tests
BDB Bioscience · Cat: 567788
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Description
Two-color flow cytometric analysis of CD206 MMR expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF Cat. No. 550068 . The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219/564220 , and stained with FITC Mouse Anti-Human CD11c antibody Cat. No. 561355 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or with BD Horizon BV421 Mouse Anti-Human CD206 MMR antibody Cat. No. 566885; Right Plot . The flow cytometric contour plot showing the correlated expression of CD206 MMR or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light scatter characteristics of viable GM-CSF-activated monocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of CD206 MMR expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF Cat. No. 550068 . The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219/564220 , and stained with FITC Mouse Anti-Human CD11c antibody Cat. No. 561355 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or with BD Horizon BV421 Mouse Anti-Human CD206 MMR antibody Cat. No. 566885; Right Plot . The flow cytometric contour plot showing the correlated expression of CD206 MMR or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light scatter characteristics of viable GM-CSF-activated monocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of CD206 MMR expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF Cat. No. 550068 . The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219/564220 , and stained with FITC Mouse Anti-Human CD11c antibody Cat. No. 561355 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or with BD Horizon BV421 Mouse Anti-Human CD206 MMR antibody Cat. No. 566885; Right Plot . The flow cytometric contour plot showing the correlated expression of CD206 MMR or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light scatter characteristics of viable GM-CSF-activated monocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Two-color flow cytometric analysis of CD206 MMR expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF Cat. No. 550068 . The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219/564220 , and stained with FITC Mouse Anti-Human CD11c antibody Cat. No. 561355 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or with BD Horizon BV421 Mouse Anti-Human CD206 MMR antibody Cat. No. 566885; Right Plot . The flow cytometric contour plot showing the correlated expression of CD206 MMR or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light scatter characteristics of viable GM-CSF-activated monocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Two-color flow cytometric analysis of CD206 MMR expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF Cat. No. 550068 . The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219/564220 , and stained with FITC Mouse Anti-Human CD11c antibody Cat. No. 561355 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or with BD Horizon BV421 Mouse Anti-Human CD206 MMR antibody Cat. No. 566885; Right Plot . The flow cytometric contour plot showing the correlated expression of CD206 MMR or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light scatter characteristics of viable GM-CSF-activated monocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Two-color flow cytometric analysis of CD206 MMR expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF Cat. No. 550068 . The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219/564220 , and stained with FITC Mouse Anti-Human CD11c antibody Cat. No. 561355 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or with BD Horizon BV421 Mouse Anti-Human CD206 MMR antibody Cat. No. 566885; Right Plot . The flow cytometric contour plot showing the correlated expression of CD206 MMR or Ig Isotype control staining versus CD11c was derived from gated events with the forward and side light scatter characteristics of viable GM-CSF-activated monocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software.