BD HorizonTM BV421 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated Left Plots and activated Right Plot human lymphocytes. Left Plots Whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486 as indicated. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot Peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on unstimulated Left Plots and activated Right Plot human lymphocytes. Left Plots Whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486 as indicated. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot Peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on unstimulated Left Plots and activated Right Plot human lymphocytes. Left Plots Whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486 as indicated. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot Peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD25 expression on unstimulated Left Plots and activated Right Plot human lymphocytes. Left Plots Whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486 as indicated. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot Peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD25 expression on unstimulated Left Plots and activated Right Plot human lymphocytes. Left Plots Whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486 as indicated. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot Peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on unstimulated Left Plots and activated Right Plot human lymphocytes. Left Plots Whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486 as indicated. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot Peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD25 antibody Cat. No. 567485/567486; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.