BD HorizonTM BV421 Mouse Anti-Human CD279 (PD-1)

Description

Multicolor flow cytometric analysis of CD279 expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 562516/565935 and APC Mouse Anti-Human CD3 antibody Cat. No. 561810/555335 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric contour plot shows the correlated expression of CD3 versus CD279 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Quadrant markers were placed based upon the corresponding fluorescent immunoglobulin isotype controls data not shown . Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Multicolor flow cytometric analysis of CD279 expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 562516/565935 and APC Mouse Anti-Human CD3 antibody Cat. No. 561810/555335 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric contour plot shows the correlated expression of CD3 versus CD279 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Quadrant markers were placed based upon the corresponding fluorescent immunoglobulin isotype controls data not shown . Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD279 expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 562516/565935 and APC Mouse Anti-Human CD3 antibody Cat. No. 561810/555335 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric contour plot shows the correlated expression of CD3 versus CD279 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Quadrant markers were placed based upon the corresponding fluorescent immunoglobulin isotype controls data not shown . Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System.