BD HorizonTM BV421 Mouse Anti-Human CD314 (NKG2D)

Description

Two-color flow cytometric analysis of CD314 NKG2D expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 Cat. No. 555516 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 568106; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD314 NKG2D expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 Cat. No. 555516 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 568106; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD314 NKG2D expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 Cat. No. 555516 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 568106; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD314 NKG2D expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 Cat. No. 555516 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 568106; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD314 NKG2D expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 Cat. No. 555516 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 568106; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD314 NKG2D expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 Cat. No. 555516 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human CD314 NKG2D antibody Cat. No. 568106; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD314 NKG2D or Ig Isotype control staining versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.