BD HorizonTM BV421 Mouse Anti-Human CD34

Description

Multiparameter flow cytometric analysis of CD34 expression on Human CD14-negative peripheral blood mononuclear cells PBMC . Human PBMC were stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CD34 antibody Cat No. 568758, Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD14-negative PBMC. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD34 expression on Human CD14-negative peripheral blood mononuclear cells PBMC . Human PBMC were stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CD34 antibody Cat No. 568758, Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD14-negative PBMC. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD34 expression on Human CD14-negative peripheral blood mononuclear cells PBMC . Human PBMC were stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CD34 antibody Cat No. 568758, Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD14-negative PBMC. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD34 expression on Human CD14-negative peripheral blood mononuclear cells PBMC . Human PBMC were stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CD34 antibody Cat No. 568758, Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD14-negative PBMC. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD34 expression on Human CD14-negative peripheral blood mononuclear cells PBMC . Human PBMC were stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CD34 antibody Cat No. 568758, Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD14-negative PBMC. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD34 expression on Human CD14-negative peripheral blood mononuclear cells PBMC . Human PBMC were stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Plot or BD Horizon BV421 Mouse Anti-Human CD34 antibody Cat No. 568758, Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative CD14-negative PBMC. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.