BD HorizonTM BV421 Mouse Anti-Human CD39

Description

Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either BD Horizon BV421 Mouse Anti-Human CD39 antibody Cat. No. 563679; solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram Left Panel . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either BD Horizon BV421 Mouse Anti-Human CD39 antibody Cat. No. 563679; solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram Left Panel . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either BD Horizon BV421 Mouse Anti-Human CD39 antibody Cat. No. 563679; solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram Left Panel . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either BD Horizon BV421 Mouse Anti-Human CD39 antibody Cat. No. 563679; solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram Left Panel . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either BD Horizon BV421 Mouse Anti-Human CD39 antibody Cat. No. 563679; solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram Left Panel . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , PE-Cy7 Mouse Anti-Human CD25 Cat. No. 557741/560920/561405 , Alexa Fluor 647 Mouse Anti-Human CD127 Cat No. 558598/560905 antibodies, and either BD Horizon BV421 Mouse Anti-Human CD39 antibody Cat. No. 563679; solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram Left Panel . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.