BD HorizonTM BV421 Mouse Anti-Human CD69

Description

Flow cytometric analysis of CD69 expression on stimulated Rhesus peripheral blood lymphocytes. Rhesus peripheral blood mononuclear cells were stimulated overnight with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with the BD Horizon BV421 Mouse Anti-Human CD69 antibody Cat. No. 562883/562884; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD69 expression on stimulated Rhesus peripheral blood lymphocytes. Rhesus peripheral blood mononuclear cells were stimulated overnight with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with the BD Horizon BV421 Mouse Anti-Human CD69 antibody Cat. No. 562883/562884; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD69 expression on stimulated Rhesus peripheral blood lymphocytes. Rhesus peripheral blood mononuclear cells were stimulated overnight with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with the BD Horizon BV421 Mouse Anti-Human CD69 antibody Cat. No. 562883/562884; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.