
570950 · 100 Tests
BDB Bioscience · Cat: 570950
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD79a expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD79a antibody Cat. No. 570950/571021; solid line histogram . The fluorescence histogram showing CD79a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD79a expression in Human peripheral mononuclear cells. Human peripheral mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human CD79a antibody Cat. No. 570950/571021; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD79a or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD79a expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD79a antibody Cat. No. 570950/571021; solid line histogram . The fluorescence histogram showing CD79a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD79a expression in Human peripheral mononuclear cells. Human peripheral mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human CD79a antibody Cat. No. 570950/571021; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD79a or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD79a expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; dashed line histogram or BD Horizon BV421 Mouse Anti-Human CD79a antibody Cat. No. 570950/571021; solid line histogram . The fluorescence histogram showing CD79a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD79a expression in Human peripheral mononuclear cells. Human peripheral mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were subsequently stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 569394; Left Plot or BD Horizon BV421 Mouse Anti-Human CD79a antibody Cat. No. 570950/571021; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD79a or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact PBMC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.