BD HorizonTM BV421 Mouse Anti-Human CD83
562630 · 50 Tests
BDB Bioscience · Cat: 562630
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/mL of Recombinant Human IL-4 Cat. No. 554605 , 20 ng/mL Recombinant Human TNF Cat. No. 554618 and 20 ng/mL Recombinant Human GM-CSF Cat. No. 550068 for 7 days at 37C. The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with the BD Horizon BV421 Mouse Anti-Human CD83 antibody Cat. No. 562630; solid line histogram . Flow cytometric histograms were derived from gated events based on the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/mL of Recombinant Human IL-4 Cat. No. 554605 , 20 ng/mL Recombinant Human TNF Cat. No. 554618 and 20 ng/mL Recombinant Human GM-CSF Cat. No. 550068 for 7 days at 37C. The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with the BD Horizon BV421 Mouse Anti-Human CD83 antibody Cat. No. 562630; solid line histogram . Flow cytometric histograms were derived from gated events based on the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/mL of Recombinant Human IL-4 Cat. No. 554605 , 20 ng/mL Recombinant Human TNF Cat. No. 554618 and 20 ng/mL Recombinant Human GM-CSF Cat. No. 550068 for 7 days at 37C. The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histogram or with the BD Horizon BV421 Mouse Anti-Human CD83 antibody Cat. No. 562630; solid line histogram . Flow cytometric histograms were derived from gated events based on the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System.