BD HorizonTM BV421 Mouse Anti-Human CXCR6 (CD186)

Description

Two-color flow cytometric analysis of CXCR6 CD186 expression on human peripheral blood cells. Human peripheral blood cells were freshly stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555342/561804 and either BD Horizon BV421 Anti-Human CXCR6 CD186 Cat. No. 566007/566008 or BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color contour plots showing the correlated expression of CXCR6 CD186 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Flow cytometric analysis of CXCR6 CD186 expression on IL-2-stimulated lymphocytes. Human PBMC were cultured 9 days with Recombinant Human IL-2 Cat. No. 554603 . Cells were stained with BD Horizon Fixable Viability Stain 660 FVS660; Cat. No. 564405 for exclusion of dead cells and then with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control dashed line histogram or BD Horizon BV421 Anti-Human CXCR6 CD186 antibody solid line histogram . The histograms showing CXCR6 CD186 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable FVS660-negative lymphocytes using a BD LSRFortessa X-20 Cell Analyzer System. Two-color flow cytometric analysis of CXCR6 CD186 expression on human peripheral blood cells. Human peripheral blood cells were freshly stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555342/561804 and either BD Horizon BV421 Anti-Human CXCR6 CD186 Cat. No. 566007/566008 or BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color contour plots showing the correlated expression of CXCR6 CD186 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Flow cytometric analysis of CXCR6 CD186 expression on IL-2-stimulated lymphocytes. Human PBMC were cultured 9 days with Recombinant Human IL-2 Cat. No. 554603 . Cells were stained with BD Horizon Fixable Viability Stain 660 FVS660; Cat. No. 564405 for exclusion of dead cells and then with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control dashed line histogram or BD Horizon BV421 Anti-Human CXCR6 CD186 antibody solid line histogram . The histograms showing CXCR6 CD186 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable FVS660-negative lymphocytes using a BD LSRFortessa X-20 Cell Analyzer System. Show More Two-color flow cytometric analysis of CXCR6 CD186 expression on human peripheral blood cells. Human peripheral blood cells were freshly stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555342/561804 and either BD Horizon BV421 Anti-Human CXCR6 CD186 Cat. No. 566007/566008 or BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color contour plots showing the correlated expression of CXCR6 CD186 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Flow cytometric analysis of CXCR6 CD186 expression on IL-2-stimulated lymphocytes. Human PBMC were cultured 9 days with Recombinant Human IL-2 Cat. No. 554603 . Cells were stained with BD Horizon Fixable Viability Stain 660 FVS660; Cat. No. 564405 for exclusion of dead cells and then with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control dashed line histogram or BD Horizon BV421 Anti-Human CXCR6 CD186 antibody solid line histogram . The histograms showing CXCR6 CD186 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable FVS660-negative lymphocytes using a BD LSRFortessa X-20 Cell Analyzer System.