BD HorizonTM BV421 Mouse Anti-Human Cytokeratin

Description

Flow cytometric analysis of cytokeratin expression in human SK-BR-3 cells. Cells from the human SK-BR-3 Breast adenocarcinoma, ATCC HTB-30 cell line were incubated with 1X BD FACS Lysing Solution Cat. No. 349202 , permeabilized with BD FACS Permeabilizing Solution 2 Cat. No. 340973/347692 , and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram or BD Horizon BV421 Mouse Anti-Human Cytokeratin antibody Cat. No. 564709; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of cytokeratin expression in human SK-BR-3 cells. Cells from the human SK-BR-3 Breast adenocarcinoma, ATCC HTB-30 cell line were incubated with 1X BD FACS Lysing Solution Cat. No. 349202 , permeabilized with BD FACS Permeabilizing Solution 2 Cat. No. 340973/347692 , and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram or BD Horizon BV421 Mouse Anti-Human Cytokeratin antibody Cat. No. 564709; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of cytokeratin expression in human SK-BR-3 cells. Cells from the human SK-BR-3 Breast adenocarcinoma, ATCC HTB-30 cell line were incubated with 1X BD FACS Lysing Solution Cat. No. 349202 , permeabilized with BD FACS Permeabilizing Solution 2 Cat. No. 340973/347692 , and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram or BD Horizon BV421 Mouse Anti-Human Cytokeratin antibody Cat. No. 564709; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.