BD HorizonTM BV421 Mouse Anti-Human GARP

Description

Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The activated PBMC were washed and stained with Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748, Left Panel or BD Horizon BV421 Mouse Anti-Human GARP antibody Cat. No. 563956/565969, Right Panel . The cells were then washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with PE Mouse Anti-Human FoxP3 antibody Cat. No. 560046/560082 . Two-color flow cytometric dot plots showing the correlated expression of FoxP3 versus GARP or Ig Isotype control staining were derived from CD4-positive events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The activated PBMC were washed and stained with Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748, Left Panel or BD Horizon BV421 Mouse Anti-Human GARP antibody Cat. No. 563956/565969, Right Panel . The cells were then washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with PE Mouse Anti-Human FoxP3 antibody Cat. No. 560046/560082 . Two-color flow cytometric dot plots showing the correlated expression of FoxP3 versus GARP or Ig Isotype control staining were derived from CD4-positive events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The activated PBMC were washed and stained with Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748, Left Panel or BD Horizon BV421 Mouse Anti-Human GARP antibody Cat. No. 563956/565969, Right Panel . The cells were then washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with PE Mouse Anti-Human FoxP3 antibody Cat. No. 560046/560082 . Two-color flow cytometric dot plots showing the correlated expression of FoxP3 versus GARP or Ig Isotype control staining were derived from CD4-positive events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The activated PBMC were washed and stained with Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748, Left Panel or BD Horizon BV421 Mouse Anti-Human GARP antibody Cat. No. 563956/565969, Right Panel . The cells were then washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with PE Mouse Anti-Human FoxP3 antibody Cat. No. 560046/560082 . Two-color flow cytometric dot plots showing the correlated expression of FoxP3 versus GARP or Ig Isotype control staining were derived from CD4-positive events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The activated PBMC were washed and stained with Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748, Left Panel or BD Horizon BV421 Mouse Anti-Human GARP antibody Cat. No. 563956/565969, Right Panel . The cells were then washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with PE Mouse Anti-Human FoxP3 antibody Cat. No. 560046/560082 . Two-color flow cytometric dot plots showing the correlated expression of FoxP3 versus GARP or Ig Isotype control staining were derived from CD4-positive events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of GARP expressed on activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 3 days with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The activated PBMC were washed and stained with Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748, Left Panel or BD Horizon BV421 Mouse Anti-Human GARP antibody Cat. No. 563956/565969, Right Panel . The cells were then washed, fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with PE Mouse Anti-Human FoxP3 antibody Cat. No. 560046/560082 . Two-color flow cytometric dot plots showing the correlated expression of FoxP3 versus GARP or Ig Isotype control staining were derived from CD4-positive events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.