
567425 · 25 Tests
BDB Bioscience · Cat: 567425
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV421 IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human HLA-E antibody Cat. No. 567424/567425; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV421 IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human HLA-E antibody Cat. No. 567424/567425; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV421 IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human HLA-E antibody Cat. No. 567424/567425; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software Show More Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV421 IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human HLA-E antibody Cat. No. 567424/567425; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software Show More Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV421 IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human HLA-E antibody Cat. No. 567424/567425; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV421 IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD Horizon BV421 Mouse Anti-Human HLA-E antibody Cat. No. 567424/567425; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software