

565940 · 25 Tests
BDB Bioscience · Cat: 565940
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human IgD antibody Cat. No. 562518/565940; solid line histogram and APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two color flow cytometric dot plots show the correlated expression of IgD versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Quadrant markers were placed based upon the corresponding fluorescent immunoglobulin isotype controls data not shown . Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Multicolor flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human IgD antibody Cat. No. 562518/565940; solid line histogram and APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two color flow cytometric dot plots show the correlated expression of IgD versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Quadrant markers were placed based upon the corresponding fluorescent immunoglobulin isotype controls data not shown . Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Show More Multicolor flow cytometric analysis of IgD expression on human peripheral blood lymphocytes. Human whole blood was stained with the BD Horizon BV421 Mouse Anti-Human IgD antibody Cat. No. 562518/565940; solid line histogram and APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two color flow cytometric dot plots show the correlated expression of IgD versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Quadrant markers were placed based upon the corresponding fluorescent immunoglobulin isotype controls data not shown . Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System.