BD HorizonTM BV421 Mouse Anti-Human IL-17A

Description

Multicolor flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Panel or with the BD Horizon BV421 Mouse Anti-Human IL-17A antibody Cat No. 562933, Right Panel in conjunction with an Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Panel or with the BD Horizon BV421 Mouse Anti-Human IL-17A antibody Cat No. 562933, Right Panel in conjunction with an Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Panel or with the BD Horizon BV421 Mouse Anti-Human IL-17A antibody Cat No. 562933, Right Panel in conjunction with an Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Multicolor flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Panel or with the BD Horizon BV421 Mouse Anti-Human IL-17A antibody Cat No. 562933, Right Panel in conjunction with an Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Multicolor flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Panel or with the BD Horizon BV421 Mouse Anti-Human IL-17A antibody Cat No. 562933, Right Panel in conjunction with an Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of IL-17A expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either a BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, Left Panel or with the BD Horizon BV421 Mouse Anti-Human IL-17A antibody Cat No. 562933, Right Panel in conjunction with an Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 557707 . Two-color flow cytometric dot plots showing the expression of IL-17A or Ig Isotype Control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometry System.