BD HorizonTM BV421 Mouse Anti-Human IL-21

Description

Two-color flow cytometric analysis of IL-21 expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD4 antibody Cat.No. 561841/555349/561840 and either BD Horizon BV421 Mouse IgG1 kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human IL-21 antibody Cat. No. 564755; Right Panel using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric contour plots showing the correlated expression of IL-21 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-21 expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD4 antibody Cat.No. 561841/555349/561840 and either BD Horizon BV421 Mouse IgG1 kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human IL-21 antibody Cat. No. 564755; Right Panel using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric contour plots showing the correlated expression of IL-21 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-21 expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD4 antibody Cat.No. 561841/555349/561840 and either BD Horizon BV421 Mouse IgG1 kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human IL-21 antibody Cat. No. 564755; Right Panel using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric contour plots showing the correlated expression of IL-21 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-21 expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD4 antibody Cat.No. 561841/555349/561840 and either BD Horizon BV421 Mouse IgG1 kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human IL-21 antibody Cat. No. 564755; Right Panel using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric contour plots showing the correlated expression of IL-21 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-21 expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD4 antibody Cat.No. 561841/555349/561840 and either BD Horizon BV421 Mouse IgG1 kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human IL-21 antibody Cat. No. 564755; Right Panel using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric contour plots showing the correlated expression of IL-21 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-21 expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD4 antibody Cat.No. 561841/555349/561840 and either BD Horizon BV421 Mouse IgG1 kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human IL-21 antibody Cat. No. 564755; Right Panel using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric contour plots showing the correlated expression of IL-21 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.